Figure 6From: Increased anxiety in corticotropin-releasing factor type 2 receptor-null mice requires recent acute stress exposure and is associated with dysregulated serotonergic activity in limbic brain areasSerotonergic and corticosteroid receptor expression are regulated differentially in response to stress in CRFR2-null mice. (A) mRNA expression of 5-HT1AR in the (B) dorsal raphe nucleus (DRN) and (C) median raphe nucleus (MRN); (D) tryptophan hydroxylase 2 (TPH2) mRNA in (E) DRN and (F) MRN; (G) serotonin transporter (SERT) protein expression in (H) hippocampal CA1, (I) CA2 and (J,K) subiculum (S); (L) glucocorticoid receptor (GR) mRNA expression in (M) CA1, (N) CA2 and (O,P) the paraventricular nucleus (PVN). Data presented as mean ± SEM for mRNA levels or densitometry signal (SERT). N = 6 to 8. ANOVA critical F(2,36) value = 3.259 for P ≤ 0.05, 5.248 for P ≤ 0.01, 8.420 for P ≤ 0.001. #P < 0.05, ##P < 0.01, ###P < 0.001 for effect of stress across genotypes. *P < 0.05, **P < 0.01, ***P < 0.001 ~P <0.10 in post-hoc analysis. ARS increased CRFR2 mRNA expression in whole brain of mice at all times points up to 48 h post-stress (Q), whereas CVMS decreased CRFR2 expression 7 days after the end of the CVMS protocol (R) . Data presented as mean ± SEM. N = 8. ANOVA critical F(5,42) value = 2.438 for P ≤ 0.05, 3.488 for P ≤ 0.01. *P < 0.05, ** P < 0.01 as compared with 0 hours in post-hoc analysis or with control group.Back to article page