The effects of early handling (EH) and single prolonged stress on mean relative glucocorticoid levels in the (A) hippocampus and (B) prefrontal cortex. (C,D) Scatter plots showing individual data points from animals included in this experiment. *P <0.05. AFR: Animal facility reared; EH: Early handling; SPS: Single prolonged stress; Con: Control; GR: Glucocorticoid receptor; Arp: Actin related protein; I.I.: Integrated intensity.